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Hi aOf course! Here’s a text-based flowchart you can directly copy into PowerPoint and then use SmartArt → Process → Vertical (or just text boxes + arrows) to style it:
[ Design & Preparation ]
↓
[ Gibson Assembly ]
↓
[ Transformation into E. coli ]
↓
[ Plasmid Preparation ]
↓
[ Transformation into Agrobacterium ]
↓
[ Plant Transformation (Floral Dip) ]
↓
[ Seed Collection & Screening ]
Each box represents a step:
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Design & Preparation → primers, vector backbone
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Gibson Assembly → DNA fragment assembly
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Transformation into E. coli → colony selection, sequencing confirmation
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Plasmid Preparation → grow clone, plasmid extraction
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Transformation into Agrobacterium → electroporation, antibiotic selection
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Plant Transformation (Floral Dip) → dip flowers, seed set
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Seed Collection & Screening → harvest & select transgenics
Would you like me to make a horizontal flowchart version too (good for slides where space is wide instead of tall)?
For digitial print visit here

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